The instrument is designed to explore a fluorescence decay of dim samples. Since the typical time scale of the fluorescence phenomenon spans over the range of 10^-8 s, a much faster setup is required to observe such a quick process. The examined sample is excited with the Picosecond Laser pulse. After deexcitation, the system measures the time of flight of a single photon emitted by the sample. Photon flies through the Monochromator and reaches the Photomultiplier. The statistics of the times of flight evaluated for each photon captured, reconstructs the shape of the fluorescence pulse induced in the sample.
A crucial requirement for a proper FPC-TDC Spectrofluorometer performance is a low probability (<10% per pulse) that the photon reaches the detector. Thus, the instrument is peerless in investigating materials with a low fluorescence yield. System is adapted to examine electrochemical samples.

Quantities measured by the instrument:

• Fluorescence time decay in relation to wavelength of light and polarization potential (in case of electro-chemical sample),
• Emissive fluorescence spectrum,
• I-V characteristics,
• Open Circuit Potential (OCP),
• Current flowing through the sample.

The system consists of:

1. Sample Chamber,
2. Picosecond Laser,
3. Monochromator for UV, VIS and IR range,
4. Photomultiplier,
5. Potentiostat,
6. Electrochemical Cuvette with platinum electrode and reference Ag/AgCl electrode,
7. Time to Digital Converter.

Sample Chamber Interior

Picosecond Laser

• Wavelengths: 405 nm, 450-460 nm, 630-640 nm,
• Half width of the pulse duration: 150 ÷ 200 ps,
• Maximum pulse repetition frequency: 1MHz,
• Light power of a pulse: 60 mW,
• Trigger type: TTL up to 1 MHz,
• Power supply: 12 VDC,

Monochromator

• Spectral range: 200 ÷ 1200 nm,
• Motorized slits, controlled via software,
• Entrance and output slit opening range: 0 ÷ 5 mm,
• Motorized internal shutter that provides full cut off of the light beam, controlled via software,

Photomultiplier

R4998 Hammamatsu

•  Anode to Cathode voltage range: up to 2500 V,
•  Spectral range: 300 ÷ 650 nm,
• Transit time spread (FWHM): 160 ps,
• Rise time: 0.7 ns.

Green marker fluorescence excited with 405 nm laser.

该仪器专为研究低强度样品的荧光衰减而设计。由于荧光现象的典型时间尺度在 10^-8 秒范围内，因此需要更快速的设备来观察如此迅速的过程。被测样品由皮秒激光脉冲激发。样品去激发后，系统测量单个光子从样品发射后的飞行时间。光子通过单色仪后到达光电倍增管。对捕获的每个光子的飞行时间进行统计分析，可重建样品中诱导产生的荧光脉冲形状。

为了确保 FPC-TDC 光谱荧光仪的性能，关键要求是光子到达探测器的概率低于 10%（每脉冲）。因此，该仪器在研究低荧光产率的材料方面具有无可匹敌的优势。系统还适配于研究电化学样品。

仪器测量的量

• 与光波长和极化电位（针对电化学样品）相关的荧光时间衰减
• 发射荧光光谱
• 电流-电压特性（I-V 特性）
• 开路电位（OCP）
• 流经样品的电流

系统包括：

1. 样品腔室，
2. 皮秒激光器，
3. 单色仪，用于紫外、可见光和红外范围，
4. 光电倍增管，
5. 恒电位仪，
6. 电化学比色皿，配有铂电极和 Ag/AgCl 参比电极，
7. 时间数字转换器。

样品室内部

皮秒激光器

• 波长：405 nm、450-460 nm、630-640 nm，
• 脉冲持续时间的半宽度：150 ÷ 200 ps，
• 最大脉冲重复频率：1MHz，
• 脉冲光功率：60 mW，
• 触发类型：TTL，最高 1 MHz，
• 电源：12 VDC，

单色仪

• 光谱范围：200 ÷ 1200 nm，
• 电动狭缝，通过软件控制，
• 入口和出口狭缝开口范围：0 ÷ 5 mm，
• 电动内置快门，可完全切断光束，通过软件控制，

光电倍增管

R4998 Hammamatsu

• 阳极至阴极电压范围：最高 2500 V，
• 光谱范围：300 ÷ 650 nm，
• 传输时间扩展（FWHM）：160 ps，
• 上升时间：0.7 ns。

405 nm 激光激发的绿色标记荧光。